Qiyin Fang (McMaster University, Canada)

Fast and Fluorescent Multiplexing Confocal Fluorescence Lifetime Imaging

In confocal microscopy, a single foci is raster scanned to form a 2-dimension or 3-dimension image. To speed up such slow scan process, multiple foci can be used, e.g. through the use of a spinning disc. We developed a new multiplexing confocal technique that scans a 2D foci array only on the target but not on the imager. Such methods allow the use of next generation discrete CMOS detector arrays and can achieves high throughput without sacrificing resolution, ideal for drug screening applications.

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